天堂VA欧美ⅤA亚洲VA老司机,久久久久亚洲AV无码专区,把女人弄爽特黄A大片视频,CHINESE猛男自慰GV网站

技術文章 / article 您的位置:網(wǎng)站首頁 > 技術文章 > TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

產(chǎn)品列表

PROUCTS LIST

TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

發(fā)布時間: 2020-05-12  點擊次數(shù): 1849次

英國TwistDx公司(前身為ASM科技有限公司)成立于1999年,基于英國劍橋Babraham研究院建立的生物技術公司。該公司通過突破等溫DNA擴增,開發(fā)的重組酶聚合酶擴增*技術(RPA),被譽為DNA診斷領域的革命性創(chuàng)新。

RT試劑盒舉例

(1)Revert Reverse Transcriptase(200U/uL)(Thermo EP0441)

(2)RNA Inhibitor (40,000 U/mL)( Enzymatics Y9240L

TWISTDX廠家回復

  “We recommend using similar RT amounts to that of a PCR reaction of the same volume, according to your chosen manufacturer’s instructions.

  We also suggest that you run reactions at 40°C and delay agitation of the reaction by a minute to give the RT time to work, otherwise you just run the reaction as you would a normal TwistAmp® reaction (it’s a one-step process).

  It’s also advisable to add RNase Inhibitor to any reaction where RNA is the target material. Any commercially available RNase Inhibitor will be suitable.

  If the M-MLV RT that you are using comes with a separate dilution/reaction buffer, we suggest not using it as it will lower the concentration of the enzyme.

  The “delay agitation of the reaction by a minute” refers to the first mixing step per the protocol in the manual (Step 4). So wait 5 minutes instead of 4 minutes before “vortex, spin down briefly, and return the samples to the reader ensuring that the tubes are returned to their original positions in the block.”

  If we were to try and use it again, we would do a titration to determine optimal quantity to add in the new formulation reactions (we haven’t used it since we changed the formulation of the reactions a couple of years ago).

  When using RNase Inhibitor. We have used 1µg /50µl reaction, some R&D users have used 2µg /50ul, which didn’t appear to have a negative effect on the performance in our own assay.

  For this : RNAse Inhibitor, that manufacture uses 10 ul of the RNAse solution in 50 ul reaction volumes for activity determination, but your customer can try different amounts 5ul or 20ul to see what will give the best results. For this product, references of 50 ul PCR reaction volumes would also be reasonable.

TwistDx以此技術為基礎生產(chǎn)的核酸擴增類產(chǎn)品,能在恒定且較低的溫度下,僅用10~20分鐘,進行單分子核酸檢測。這一技術對環(huán)境、硬件設施的要求很低,對于生物防護、水體檢驗、食品檢驗、醫(yī)療診斷、微流體、獸醫(yī)等方面都有良好的應用。

RPA擴增的各種體系試劑盒中,含有DNA、RNA擴增所需的所有試劑,研究者只要準備好引物和模板就行了。擴增結果可以分別通過凝膠電泳檢測、熒光定量檢測或試紙條檢測,產(chǎn)物純化后可用于下游研究。

默瑞生物

默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學產(chǎn)品推廣與服務商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務體系,提升客戶體驗,以莫納生物產(chǎn)品為主線,結合國內(nèi)外優(yōu)質產(chǎn)品提供高標準完整解決方案,幫助科研客戶和生物技術企業(yè)高效創(chuàng)新,加速轉化,創(chuàng)造更多優(yōu)質產(chǎn)品,提升社會福祉,實現(xiàn)多方共贏。

  默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導入TwistDx,enzymatics,KAPABIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質品牌,逐步培育成為專業(yè)用戶的選擇。

在線客服 聯(lián)系方式

服務熱線

17317196276

国产无遮挡又黄又爽在线观看| 无码人妻精品一区二区三区蜜臀| 久久欧美人人做人人爱| А天堂中文地址在线| 狠狠人妻久久久久久综合| 顶级欧美色妇XXXXX| 亚洲A∨无码一区二区三区| 国产肉体XXXX裸体784大胆 | 99久久国产精品免费热7788| 人人妻人人澡人人爽国产| 女性扒开大腿露出私密部位| 中文亚洲AV片在线观看无码| 美女浴室洗澡裸体爆乳无遮挡| 最近免费观看高清韩国日本大全| 色婷婷亚洲精品综合影院| 囯产精品一品二区三区| 精品久久久久久久久亚洲偷窥女厕 | 日韩人妻无码一区二区三区综合部| 国产日产亚洲系列最新| 久久久精品妓女影院妓女网| 大肉大捧一进一出视频| Z0ZOZ0另类Z0ZO| 无码欧亚熟妇人妻AV在线| 少妇AAA级久久久无码精品片| 99久久久免费精品国产| 成年男人露JIJI网站自慰 | 日韩精品人妻一区二区三区四区| 色噜噜狠狠色综合成人网| 性少妇VIDEOXXX欧美69 | 内衣柜办公室L~3带樱花| 国产97在线 | 亚洲| 体育老师在单杠C了我一节课| 久久WWW免费人成一看片| 人妻丰满熟妇AV无码区乱| 永久免费观看播放器的软件| 日本无翼乌邪恶大全彩H下拉式 | 曰批全过程免费视频观看软件下载 | 宝贝的小馒头长大了| 人人妻人人澡人人爽精品欧美| 久久久精品人妻一区二区三区四| 国内少妇偷人精品视频免费|