天堂VA欧美ⅤA亚洲VA老司机,久久久久亚洲AV无码专区,把女人弄爽特黄A大片视频,CHINESE猛男自慰GV网站

技術文章 / article 您的位置:網(wǎng)站首頁 > 技術文章 > TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

產(chǎn)品列表

PROUCTS LIST

TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

發(fā)布時間: 2020-05-12  點擊次數(shù): 1849次

英國TwistDx公司(前身為ASM科技有限公司)成立于1999年,基于英國劍橋Babraham研究院建立的生物技術公司。該公司通過突破等溫DNA擴增,開發(fā)的重組酶聚合酶擴增*技術(RPA),被譽為DNA診斷領域的革命性創(chuàng)新。

RT試劑盒舉例

(1)Revert Reverse Transcriptase(200U/uL)(Thermo EP0441)

(2)RNA Inhibitor (40,000 U/mL)( Enzymatics Y9240L

TWISTDX廠家回復

  “We recommend using similar RT amounts to that of a PCR reaction of the same volume, according to your chosen manufacturer’s instructions.

  We also suggest that you run reactions at 40°C and delay agitation of the reaction by a minute to give the RT time to work, otherwise you just run the reaction as you would a normal TwistAmp® reaction (it’s a one-step process).

  It’s also advisable to add RNase Inhibitor to any reaction where RNA is the target material. Any commercially available RNase Inhibitor will be suitable.

  If the M-MLV RT that you are using comes with a separate dilution/reaction buffer, we suggest not using it as it will lower the concentration of the enzyme.

  The “delay agitation of the reaction by a minute” refers to the first mixing step per the protocol in the manual (Step 4). So wait 5 minutes instead of 4 minutes before “vortex, spin down briefly, and return the samples to the reader ensuring that the tubes are returned to their original positions in the block.”

  If we were to try and use it again, we would do a titration to determine optimal quantity to add in the new formulation reactions (we haven’t used it since we changed the formulation of the reactions a couple of years ago).

  When using RNase Inhibitor. We have used 1µg /50µl reaction, some R&D users have used 2µg /50ul, which didn’t appear to have a negative effect on the performance in our own assay.

  For this : RNAse Inhibitor, that manufacture uses 10 ul of the RNAse solution in 50 ul reaction volumes for activity determination, but your customer can try different amounts 5ul or 20ul to see what will give the best results. For this product, references of 50 ul PCR reaction volumes would also be reasonable.

TwistDx以此技術為基礎生產(chǎn)的核酸擴增類產(chǎn)品,能在恒定且較低的溫度下,僅用10~20分鐘,進行單分子核酸檢測。這一技術對環(huán)境、硬件設施的要求很低,對于生物防護、水體檢驗、食品檢驗、醫(yī)療診斷、微流體、獸醫(yī)等方面都有良好的應用。

RPA擴增的各種體系試劑盒中,含有DNA、RNA擴增所需的所有試劑,研究者只要準備好引物和模板就行了。擴增結果可以分別通過凝膠電泳檢測、熒光定量檢測或試紙條檢測,產(chǎn)物純化后可用于下游研究。

默瑞生物

默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學產(chǎn)品推廣與服務商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務體系,提升客戶體驗,以莫納生物產(chǎn)品為主線,結合國內(nèi)外優(yōu)質產(chǎn)品提供高標準完整解決方案,幫助科研客戶和生物技術企業(yè)高效創(chuàng)新,加速轉化,創(chuàng)造更多優(yōu)質產(chǎn)品,提升社會福祉,實現(xiàn)多方共贏。

  默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導入TwistDx,enzymatics,KAPABIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質品牌,逐步培育成為專業(yè)用戶的選擇。

在線客服 聯(lián)系方式

服務熱線

17317196276

最近中文字幕视频在线MV高清版| 地铁跑酷国际服下载| 无码人妻精品一区二区| 小雪第一次尝到又大又粗| 中文字幕在线观看| 久久久精品妓女影院妓女网| ZOOM与人性ZOOM视频| 欧美黑人又大又粗XXXXX| 中文字幕AⅤ人妻一区二区| 亚洲精品久久久无码| 国产一二三四2021精字窝| 国产96在线 | 欧美| 亚洲V欧美V国产V在线观看| 国产最新精品SM调教视频| 亚洲AV无码国产精品色重口色情| 国产亚洲精品久久久久久国模美 | 成人亚洲区无码偷拍12P| 欧美精产国品一二三产品区别| 精品一区二区三区无码AV| 人妻另类 专区 欧美 制服| 国产精品国产三级国产AV主播| 一色一伦一区二区三区| 97午夜理论片在线影院| 久久精品国产亚洲AV香蕉| 国产精品久久久久精品香蕉| 亚洲AV无码日韩AV无码网站冲| 邻居少妇张开腿让我爽了在线观看 | 久久久久久久久久国产精品免费| 久久精品人妻无码一区二区三区盗| 邻居的色诱中文字幕| 美女高潮无遮挡免费视频| 亚洲A∨无码一区二区三区| 男女一边摸一边做爽爽的免费视频| 再灬再灬再灬深一点舒服| 成人DVD碟片| 波多野结衣乳巨码无在线观看 | 久久久国产精品无码一区二区三区 | 国产精品久久久久久妇女| 第一次处破女14分钟| 成品网站W灬源码三叶草下载| 939W乳液78W78W韩国|